Coding

Part:BBa_K4297012

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


PAR4

The phenylacetaldehyde reductase PAR4 is form Y. lipolytica, which can catalyze the reaction of phenylacetaldehyde to 2-PE.

Identifying the optimal phenylacetaldehyde reductase/alcohol dehydrogenase. a) The morphology analysis of engineering strains; b) Performing the whole-cell biocatalytic conversion; c) The 2-PE titer of the whole-cell biocatalytic conversion

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
    Illegal BamHI site found at 293
    Illegal XhoI site found at 999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 423
    Illegal BsaI.rc site found at 1013


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Parameters
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